Estimation of minimal size of translocated chromosome segments detectable by fluorescence in situ hybridization

Kodama Y, Nakano M, Ohtaki K, Delongchamp RR, Awa AA, Nakamura N
Int J Radiat Biol 71(1):35-9, 1997

Summary

Apparent non-reciprocal translocations are commonly observed using fluorescence in situ hybridization. We hypothesize that these are ‘hidden’ reciprocals due to one translocated segment being too small to detect. Assuming that the translocation breakpoints distribute randomly, the proportion of reciprocal to non-reciprocal exchanges can be used to estimate the minimal detectable size of translocated segments. To estimate segment size in this study, cytogenetic data for 120 A-bomb survivors were used. Among 2295 aberrant metaphases, 1629 exhibited reciprocal translocations and 666 non-reciprocal. Of the non-reciprocal translocations, 501 showed only a painted chromosome segment, translocated to an unpainted chromosome with centromere; and 165 showed only an unpainted chromosome segment, translocated to a painted chromosome with centromere. On the basis of the above two assumptions, we obtained the most likely estimates for minimal detectable sizes: 11.1 plus or minus 0.8 Mb for the painted and 14.6 plus or minus 0.6 Mb for the unpainted chromosomes. The implications of these findings are discussed.