Technical Report No. 15-85
Effects of 60Co gamma radiaton on defense function of human polymorphonuclear leukocytes
Sasagawa S, Suzuki K, Sakatani T, Brooks GT, Fujikura TSummary
The effects of radiation on defense function of polymorphonuclear leukocytes (PMN) were studied following irradiation with 60Co gamma radiation (30-3,000 rad) using PMN separated from the peripheral blood of healthy volunteers. The migration distances for all three measures of chemotaxis to fMet-Leu-Phe (10-8 M), chemokinesis induced by fMet-Leu-Phe, and random migration tended to decrease with increasing dose, showing 0.0054 mum/rad (p < 0.001) for chemotaxis, and 0.0018 mum/rad (p = 0.005) for both chemokinesis and random migration. For releases of beta-glucuronidase (BGL) and lysozyme (LYZ) from PMN stimulated by fMet-Leu-Phe (10-5 M) in conjunction with cytochalasin B (CB, 5 mug/ml) there was a significant dose trend, showing the dose effects of decreasing 0.0022% release/rad for BGL and 0.0030% release/rad for LYZ with increasing dose. In superoxide anion production, a slight and marginally significant linear dose trend was found. These results suggest that the defense function of PMN is not so resistant to radiation as predicted from the fact that PMN in the peripheral blood are differentiated and mature. It is thought that radiation inflicts substantially harmful effects on the defense function of peripheral PMN.
The effects of radiation on defense function of polymorphonuclear leukocytes (PMN) were studied following irradiation with 60Co gamma radiation (30-3,000 rad) using PMN separated from the peripheral blood of healthy volunteers. The migration distances for all three measures of chemotaxis to fMet-Leu-Phe (10-8 M), chemokinesis induced by fMet-Leu-Phe, and random migration tended to decrease with increasing dose, showing 0.0054 mum/rad (p < 0.001) for chemotaxis, and 0.0018 mum/rad (p = 0.005) for both chemokinesis and random migration. For releases of beta-glucuronidase (BGL) and lysozyme (LYZ) from PMN stimulated by fMet-Leu-Phe (10-5 M) in conjunction with cytochalasin B (CB, 5 mug/ml) there was a significant dose trend, showing the dose effects of decreasing 0.0022% release/rad for BGL and 0.0030% release/rad for LYZ with increasing dose. In superoxide anion production, a slight and marginally significant linear dose trend was found. These results suggest that the defense function of PMN is not so resistant to radiation as predicted from the fact that PMN in the peripheral blood are differentiated and mature. It is thought that radiation inflicts substantially harmful effects on the defense function of peripheral PMN.