Technical Report No. 11-87
In vitro radiosensitivity of human fresh T-lymphocytes by colony formation assay using PHA and recombinant interleukin-2
Tatsugawa K, Nakamura N, Hakoda M, Akiyama MEditor’s note: A publication based on this report was published in J Radiat Res (Tokyo) 28:221-6, 1987.
Summary
In vitro culture conditions for colony formation of human fresh peripheral T-cells using phytohemagglutinin and recombinant Interleukin-2 are defined. Peripheral lymphocytes, from six individuals, were exposed to X or gamma rays in vitro, and dose-survival curves were obtained. The results showed typical sigmoid curves similar to those observed when other mammalian cells are exposed to radiation. The D10 (dose required to kill 90% of the cells) was found to be 3.0 to 3.5 Gy.
In vitro culture conditions for colony formation of human fresh peripheral T-cells using phytohemagglutinin and recombinant Interleukin-2 are defined. Peripheral lymphocytes, from six individuals, were exposed to X or gamma rays in vitro, and dose-survival curves were obtained. The results showed typical sigmoid curves similar to those observed when other mammalian cells are exposed to radiation. The D10 (dose required to kill 90% of the cells) was found to be 3.0 to 3.5 Gy.