Technical Report No. 11-88
Cloning of phenotypically different HPRT mutant human lymphocytes originating from a single stem cell
Hakoda M, Hirai Y, Shimba H, Kusunoki Y, Kyoizumi S, Kodama Y, Akiyama MSummary
Thioguanine-resistant T, NK, and B cells have been cloned from the peripheral blood of an atomic bomb survivor in Hiroshima. The cloned lymphocytes possessed the same chromosome aberration and the same alteration of DNA at the hypoxanthine phosphoribosyltransferase (HPRT) locus, but exhibited different surface phenotypes and different rearrangements of T cell receptor or immunoglobulin genes. These results indicate that the cloned lymphocytes originated from a single undifferentiated stem cell. The chromosome aberration in the cloned lymphocytes suggested that the original stem cell started differentiation after the A-bomb exposure (the person studied was exposed to the A-bomb at age 17). The present study has provided a novel method to investigate the differentiation of human lymphocytes using the HPRT gene alterations and chromosome aberrations as cellular markers.
Thioguanine-resistant T, NK, and B cells have been cloned from the peripheral blood of an atomic bomb survivor in Hiroshima. The cloned lymphocytes possessed the same chromosome aberration and the same alteration of DNA at the hypoxanthine phosphoribosyltransferase (HPRT) locus, but exhibited different surface phenotypes and different rearrangements of T cell receptor or immunoglobulin genes. These results indicate that the cloned lymphocytes originated from a single undifferentiated stem cell. The chromosome aberration in the cloned lymphocytes suggested that the original stem cell started differentiation after the A-bomb exposure (the person studied was exposed to the A-bomb at age 17). The present study has provided a novel method to investigate the differentiation of human lymphocytes using the HPRT gene alterations and chromosome aberrations as cellular markers.