Technical Report No. 17-89
Absence of correlations between the radiosensitivity of human T-lymphocytes at G0 and skin fibroblasts at log phase from the same individuals
Kushiro J, Nakamura N, Kyoizumi S, Nishiki M, Dohi K, Akiyama MEditor’s note: A publication based on this report was published in Radiat Res 122:326-32, 1990.
Summary
Matched samples of peripheral T-lymphocytes and skin fibroblasts from a total of 22 patients who underwent various surgical procedures were tested for a dose-survival study using loss of colony-forming ability as the end point. The results showed that the mean D10 (the dose required to kill 90% of the cells) plus or minus SD was 3.58 plus or minus 0.21 Gy for T-lymphocytes irradiated at G0 and 3.19 plus or minus 0.37 Gy for skin fibroblasts irradiated at log phase. The coefficients of variation were found to be 6% and 11%, respectively. Contrary to expectation, regression analysis of the D10 values for the two cell types revealed no significant correlations.
The absence of correlation is most probably derived from the fact that the apparent interindividual variability of dose-survival curves is largely caused by random experimental fluctuations, at least for lymphocytes. Possible reasons for the greater variability observed in the fibroblast assay are discussed.
Matched samples of peripheral T-lymphocytes and skin fibroblasts from a total of 22 patients who underwent various surgical procedures were tested for a dose-survival study using loss of colony-forming ability as the end point. The results showed that the mean D10 (the dose required to kill 90% of the cells) plus or minus SD was 3.58 plus or minus 0.21 Gy for T-lymphocytes irradiated at G0 and 3.19 plus or minus 0.37 Gy for skin fibroblasts irradiated at log phase. The coefficients of variation were found to be 6% and 11%, respectively. Contrary to expectation, regression analysis of the D10 values for the two cell types revealed no significant correlations.
The absence of correlation is most probably derived from the fact that the apparent interindividual variability of dose-survival curves is largely caused by random experimental fluctuations, at least for lymphocytes. Possible reasons for the greater variability observed in the fibroblast assay are discussed.