Technical Report No. 20-89
Estimating spontaneous mutation rates at enzyme loci in Drosophila melanogaster
Mukai T, Yamazaki T, Harada K, Kusakabe SSummary
Spontaneous mutations were accumulated for 1,620,826 allele-generations on chromosomes that originated from six stem second chromosomes of Drosophila melanogaster. Only null-electromorph mutations were detected. Band-electromorph mutations were not found. The average rate of null-electromorph mutations was 2.71 x 10-5 per locus per generation. The 95% confidence interval (mun) was 1.97 x 10-5 < mun < 3.64 x 10-5 per locus per generation. The upper 95% confidence limit of the band-electromorph mutation rate (muB) was 2.28 x 10-6 per locus per generation. It appeared that null mutations were induced by movable genetic elements and that the mutation rates were different from chromosome to chromosome.
Spontaneous mutations were accumulated for 1,620,826 allele-generations on chromosomes that originated from six stem second chromosomes of Drosophila melanogaster. Only null-electromorph mutations were detected. Band-electromorph mutations were not found. The average rate of null-electromorph mutations was 2.71 x 10-5 per locus per generation. The 95% confidence interval (mun) was 1.97 x 10-5 < mun < 3.64 x 10-5 per locus per generation. The upper 95% confidence limit of the band-electromorph mutation rate (muB) was 2.28 x 10-6 per locus per generation. It appeared that null mutations were induced by movable genetic elements and that the mutation rates were different from chromosome to chromosome.