Technical Report No. 5-90
Isolation and partial characterization of peripheral blood CD4+ T cell clones expressing gamma delta T cell receptors
Kyoizumi S, Akiyama M, Hirai Y, Kusunoki YEditor’s note: A publication based on this report was published in Immunol Letters 29:197-204, 1991.
Summary
Rare T cell clones bearing both CD4 and T cell receptors (TCR-gamma and TCR-delta) were obtained from human peripheral blood by cell sorting using anti-CD4 and anti-TCR-delta-1 antibodies. All the clones established were reactive with anti-TCR-gamma-delta-1 antibody, whereas only about 20% of the clones showed reactivity with anti-delta-TCS1 antibody. Unlike CD4+ T cells bearing TCR-alpha-beta, all the clones tested were lectin-dependent and showed CD3 antibody-redirected cytolytic activity. About 60% exhibited natural killer cell-like activity. Immunoprecipitation analysis of TCR-gamma-delta showed that each clone expressed either a disulfide-linked or nondisulfide-linked heterodimer consisting of 37-44 kilodalton TCR-gamma and TCR-delta chains. Southern blot analyses of TCR-gamma and TCR-delta genes revealed some identical rearrangement patterns, suggesting the limited heterogeneity of CD4+TCR-gamma-delta+ T cells in peripheral blood.
Rare T cell clones bearing both CD4 and T cell receptors (TCR-gamma and TCR-delta) were obtained from human peripheral blood by cell sorting using anti-CD4 and anti-TCR-delta-1 antibodies. All the clones established were reactive with anti-TCR-gamma-delta-1 antibody, whereas only about 20% of the clones showed reactivity with anti-delta-TCS1 antibody. Unlike CD4+ T cells bearing TCR-alpha-beta, all the clones tested were lectin-dependent and showed CD3 antibody-redirected cytolytic activity. About 60% exhibited natural killer cell-like activity. Immunoprecipitation analysis of TCR-gamma-delta showed that each clone expressed either a disulfide-linked or nondisulfide-linked heterodimer consisting of 37-44 kilodalton TCR-gamma and TCR-delta chains. Southern blot analyses of TCR-gamma and TCR-delta genes revealed some identical rearrangement patterns, suggesting the limited heterogeneity of CD4+TCR-gamma-delta+ T cells in peripheral blood.