Technical Report No. 12-91
Simple, rapid HLA-DQA1 genotyping using the polymerase chain reaction and analysis by single-strand conformation polymorphism and restriction-enzyme cleavage
Hayashi T, Seyama T, Ito T, Kusunoki Y, Hirai Y, Nakamura N, Akiyama MEditor’s note: A publication based on this report was published in Electrophoresis 13:877-9, 1992.
Summary
A simple and rapid method for identifying alleles at the human-leucocyte-antigen-DQA1 locus (HLA-DQA1 locus) is described. The polymorphic second exon of the HLA-DQA1 locus was amplified by the polymerase-chain-reaction method. The amplified DNA was then analyzed by the single-strand-conformation-polymorphism/restriction-enzyme-cleavage assay. Using this method, the eight known DQA1 alleles could be distinguished from each other. In this paper, this method is suggested for quick genotyping of DQA1 alleles, but this method should also be useful for detecting point mutations at various positions in a fragment as well as new HLA-DQA1 genotypes.
A simple and rapid method for identifying alleles at the human-leucocyte-antigen-DQA1 locus (HLA-DQA1 locus) is described. The polymorphic second exon of the HLA-DQA1 locus was amplified by the polymerase-chain-reaction method. The amplified DNA was then analyzed by the single-strand-conformation-polymorphism/restriction-enzyme-cleavage assay. Using this method, the eight known DQA1 alleles could be distinguished from each other. In this paper, this method is suggested for quick genotyping of DQA1 alleles, but this method should also be useful for detecting point mutations at various positions in a fragment as well as new HLA-DQA1 genotypes.