Technical Report No. 21-85
Inherited electrophoretic variants detected in a Japanese population with two-dimensional gels of erythrocyte lysates
Takahashi N, Neel JV, Shimoichi Y, Asakawa J, Tanaka Y, Satoh CEditor’s note: A publication based on this report was published in Ann Hum Genet 50:313-25, 1986.
Summary
Genetic variation has been studied in erythrocyte lysates from 100 Japanese children and their parents by means of two-dimensional polyacryl-amide gel electrophoresis (2-D PAGE). Fifty-five polypeptides selected without respect to variability were considered suitable for scoring. Genetic variation was encountered in 14 of these polypeptides. Family data show that the segregation of 13 variants is consistent with an autosomal codominant mode of inheritance; the remaining variant exhibits a sex-linked mode of inheritance. Of eight presumably identical polypeptides found to be variable in Japanese and /or Caucasians, differences in the occurrence or allele frequencies of polymorphisms were recognized for four. Contrary to the experience of some investigators, the amount of variation and the ethnic differences we are encountering indicate that 2-D PAGE is a sensitive tool for the study of genetic events.
Genetic variation has been studied in erythrocyte lysates from 100 Japanese children and their parents by means of two-dimensional polyacryl-amide gel electrophoresis (2-D PAGE). Fifty-five polypeptides selected without respect to variability were considered suitable for scoring. Genetic variation was encountered in 14 of these polypeptides. Family data show that the segregation of 13 variants is consistent with an autosomal codominant mode of inheritance; the remaining variant exhibits a sex-linked mode of inheritance. Of eight presumably identical polypeptides found to be variable in Japanese and /or Caucasians, differences in the occurrence or allele frequencies of polymorphisms were recognized for four. Contrary to the experience of some investigators, the amount of variation and the ethnic differences we are encountering indicate that 2-D PAGE is a sensitive tool for the study of genetic events.