Technical Report No. 2-87
Monoclonal antibodies KL-3 and KL-6 against human pulmonary adenocarcinoma: 1. Characterization of the antibodies and their application in detection of tumor cells in pleural effusion
Kohno N, Akiyama M, Kyoizumi S, Hakoda M, Kobuke K, Yamakido MSummary
Two monoclonal antibodies, KL-3 (IgM) and KL-6 (IgG1), were produced by the fusion of murine myeloma NS1 cells with splenocytes from a BALB/c mouse immunized with human pulmonary adenocarcinoma VMRC-LCR cells. Both antibodies recognized carbohydrate antigens. Immunohistochemical analysis of tissue sections showed that KL-3 antibodies preferentially react with lung adenocarcinomas and small cell carcinomas, whereas KL-6 antibodies react with these as well as squamous cell carcinomas of the lung. Both of these antibodies also reacted with many different types of cancer. Immunofluorescence was used to demonstrate that both antibodies were reactive only with tumor cells but not with nonmalignant blood cells and mesothelial cells in pleural effusions. These antibodies may be clinically useful in immunocytochemical diagnosis of tumor cells in pleural effusions.
Two monoclonal antibodies, KL-3 (IgM) and KL-6 (IgG1), were produced by the fusion of murine myeloma NS1 cells with splenocytes from a BALB/c mouse immunized with human pulmonary adenocarcinoma VMRC-LCR cells. Both antibodies recognized carbohydrate antigens. Immunohistochemical analysis of tissue sections showed that KL-3 antibodies preferentially react with lung adenocarcinomas and small cell carcinomas, whereas KL-6 antibodies react with these as well as squamous cell carcinomas of the lung. Both of these antibodies also reacted with many different types of cancer. Immunofluorescence was used to demonstrate that both antibodies were reactive only with tumor cells but not with nonmalignant blood cells and mesothelial cells in pleural effusions. These antibodies may be clinically useful in immunocytochemical diagnosis of tumor cells in pleural effusions.