RP 1-08

Establishment of a recombinant mouse model for assessment of genetic effects of radiation at low doses

Summary

In this project, we propose to create genetically modified mice in which mutant cells arising in their body will generate green fluorescence. By using these mice, genetic effects of radiation (transgenerational effects) will be measured in their germ cells; spermatids, spermatocytes, spermatogonia, and oocytes. This approach could pave the way to analyze genetic effects of radiation at low doses without requiring a large number of F1 animals. To study the possible negative selection of mutant embryos or neonatal mice carrying the mutation, a fraction of mutant germ cells will be used to produce fertilized eggs which will be subsequently transplanted in pseudopregnant mice’s wombs to scrutinize the developmental/growth process. Furthermore, mutant cell frequency of somatic cells in F1 animals born to irradiated mice will be measured to test if genomic instability might be observed as a delayed effect of parental exposure.

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